Journal: Arthroscopy Techniques

Article Title: Posterior Meniscal Root Repair Using a Meniscal Suture Device

doi: 10.1016/j.eats.2020.03.006

Figure Lengend Snippet: We create a lateral access of approximately 6 cm in length, identify the iliotibial tract, and section it at its posterior third; below the iliotibial band, we identified the lateral collateral ligament. We also identify the fibular head and femoral biceps. We perform digital dissection between the posterior surface of the lateral femoral condyle and the head of the lateral gastrocnemius. During arthroscopic visualization, we identify the posterior root lesion of the lateral meniscus. We prepare the Arthrex MU (multiuse) guide with the 6-mm FlipCutter drill. We realize an anterolateral approach to the proximal tibia to make the transtibial tunnel for root reinsertion. We position the MU guide on the footprint of the posterior root of the lateral meniscus. After making sure that the drill is in the correct position, we horizontalize the tip of the FlipCutter drill and make a 10-mm-deep tunnel. We then pass an inverted needled guidewire as a transport wire and pull a loop of this wire to the anterolateral portal. We identify the popliteal tendon and introduce the Meniscus 4 A-II device posterior and proximal to the popliteal tendon, extending into the proximal side of the lateral meniscus. Using tweezers, we grasp the posterior horn of the lateral meniscus and pierce it. Again using tweezers, we pull a handle out of the joint through the anterolateral portal. With the same tweezers, we pull both ends of the FiberWire out of the anterolateral portal. We then make a stitch by passing the 2 ends of the wire through a new loop formed at the end of the loop and pulling the 2 ends, causing the tip to run to the posterior horn of the lateral meniscus. We repeated the same step by passing the meniscal suture device again through the posterior horn of the lateral meniscus. We pull the handle out of the joint through the anterolateral portal. We also pull into the anterolateral portal the 2 ends of the same wire out of the posterolateral approach, making the same point described above. Care must be taken to prevent the previous suture from becoming caught in the new stitch; if this occurs, we remove the wires from the previous stitch from inside the new suture. Then, we pull all the wires simultaneously out of the transpatellar portal to avoid catching any wires in the joint capsule. After this last step, we pass the free ends of the wires through the preformed loop to be transported to the tibial tunnel. Holding them inside the joint makes it easier for them to slide into the tibial tunnel. We pull the 4 wires together, observing the lateral meniscal root reinsertion, and finally fix the wires with a button or post screw.

Article Snippet: We prepare the Meniscus 4 A-II meniscal suture device with No. 2-0 FiberWire thread (Arthrex) passed through its end folded into 2 symmetrical parts ( A).

Techniques:

Journal: ACS biomaterials science & engineering

Article Title: AFM-Nanomechanical Test: An Interdisciplinary Tool That Links the Understanding of Cartilage and Meniscus Biomechanics, Osteoarthritis Degeneration, and Tissue Engineering

doi: 10.1021/acsbiomaterials.7b00307

Figure Lengend Snippet: Hierarchical structure of articular cartilage extracellular matrix (ECM). (a) Schematic of the structure and major constituents of cartilage ECM: type II/IX/XI collagen fibril network and aggrecan–hyaluronan aggregates.18 Molecular density is reduced to increase clarity. Adapted with permission from ref 31. Copyright 2014 American Chemical Society. (b) Matrix molecular composition and organization in different extracellular regions. Adapted with permission from ref 10. Copyright 2009 Wiley. (c,d) Zonal heterogeneity: Toluidine blue histology images of rabbit cartilage showing (c) the pericellular, territorial, and interterritorial matrices; (d) depth-dependent zonal heterogeneity. Adapted with permission from ref 11. Copyright 2006 Osteoarthritis Research Society International. (e) Collagen fibril orientation distribution in relation to cartilage surface, measured by the quantitative polarized light microscopy (qPLM) on mature porcine cartilage. Adapted with permission from ref 13. Copyright 2006 Osteoarthritis Research Society International. (f) Nanostructure of collagen fibril network measured by helium ion microscopy on proteoglycan-removed rabbit cartilage. Adapted with permission from ref 14. Copyright 2012 Wiley. (g) Nanostructure of aggrecan-hyaluronan aggregates imaged by (top panel) transmission electron microscopy and (middle panel) tapping mode AFM, as well as (bottom panel) nanstructure of individual aggrecan by tapping mode AFM. Adapted with permission from refs 17 and 20. Copyright 2010 Osteoarthritis Research Society International and 1983 Gustav Fischer Verlag.

Article Snippet: 157 , 158 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7. caption a7 Nanomechanics of the meniscus ECM. (a) Schematic of the hierarchical structure of the meniscus ECM.

Techniques: Light Microscopy, Microscopy, Transmission Assay, Electron Microscopy

Journal: ACS biomaterials science & engineering

Article Title: AFM-Nanomechanical Test: An Interdisciplinary Tool That Links the Understanding of Cartilage and Meniscus Biomechanics, Osteoarthritis Degeneration, and Tissue Engineering

doi: 10.1021/acsbiomaterials.7b00307

Figure Lengend Snippet: Immunofluorescence (IF)-guided AFM nanomechanical mapping of cartilage elastic modulus. (a) Left panel: schematic of the IF-guided AFM on cryo-sectioned cartilage. Right panel: phase contrast (top) and IF images of type VI collagen illustrating the PCM and territorial/interterritorial ECM (T/IT-ECM) during AFM tests. Adapted with permission from ref 109. Copyright 2013 Elsevier Ltd. (b) Comparison of the IF-labeled adult human and murine cartilage images shows the much higher density of cells and PCM regions in the murine tissue. Adapted with permission from ref 72. Copyright 2013 Osteoarthritis Research Society International (human cartilage image only; murine cartilage image is original). (c) Left panel: representative modulus map and IF image of porcine cartilage section with a PCM terrain surrounding a cell-sized void. Right panel: elastic indentation modulus, Eind, of T/IT-ECM versus PCM in different depth-dependent zones of cartilage (mean ± SEM for 20 images per zone from n = 5 mice, *p < 0.05 between T/IT-ECM and PCM; whereas the ECM shows significant depth-dependent variations, the PCM exhibits zonal uniformity). Adapted with permission from ref 105. Copyright 2012 The Royal Society of Chemistry.

Article Snippet: 157 , 158 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7. caption a7 Nanomechanics of the meniscus ECM. (a) Schematic of the hierarchical structure of the meniscus ECM.

Techniques: Immunofluorescence, Comparison, Labeling

Journal: ACS biomaterials science & engineering

Article Title: AFM-Nanomechanical Test: An Interdisciplinary Tool That Links the Understanding of Cartilage and Meniscus Biomechanics, Osteoarthritis Degeneration, and Tissue Engineering

doi: 10.1021/acsbiomaterials.7b00307

Figure Lengend Snippet: Applications of AFM-nanoindentation on murine cartilage in the studies of development and osteoarthritis. (a) Development phenotype of cartilage T/IT-ECM and PCM in the absence of type VI collagen. Left panel: elastic modulus maps obtained via IF-guided AFM on 2-month-old wild-type (WT) and Col6a1−/− murine femoral head cartilage, and corresponding perlecan-labeled IF image. Right panel: comparison of the T/IT-ECM and PCM moduli between WT and Col6a1−/− cartilage at 2 and 9 month ages (mean ± SEM of ≥24 sites from n = 3 animals; *p < 0.05 between genotypes). Adapted with permission from ref 141. Copyright 2015 American College of Rheumatology. (b) Comparison of the high frequency modulus, EH, and hydraulic permeability, k, of 220 day old knee cartilage from WT and aggrecanase-resistant (Jaffa) mice after forced wheel running (mean ± SEM, n ≥ 3). Adapted with permission from ref 143. Copyright 2008 Elsevier Ltd. (c) Nanoindentation modulus precedes histological signs in post-traumatic osteoarthritis (PTOA) in mice. Comparison of the timelines of cartilage modulus reduction (EDMM/ESham) versus histology-based modified Mankin scores in PTOA induced by the destabilization of the medial meniscus (DMM) performed on 3 month old C57BL/6 male WT mice (mean ±95% CI, n ≥ 5). Adapted with permission from ref 145. Copyright 2016 Osteoarthritis Research Society International.

Article Snippet: 157 , 158 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7. caption a7 Nanomechanics of the meniscus ECM. (a) Schematic of the hierarchical structure of the meniscus ECM.

Techniques: Labeling, Comparison, Permeability, Modification

Journal: ACS biomaterials science & engineering

Article Title: AFM-Nanomechanical Test: An Interdisciplinary Tool That Links the Understanding of Cartilage and Meniscus Biomechanics, Osteoarthritis Degeneration, and Tissue Engineering

doi: 10.1021/acsbiomaterials.7b00307

Figure Lengend Snippet: Nanomechanics of the meniscus ECM. (a) Schematic of the hierarchical structure of the meniscus ECM. The ECM is dominated by circumferentially aligned type I collagen fiber bundles (diameter ~10–100 μm),149 which are wrapped by a ~200 μm superficial lamellar layer of radially aligned fibers,151 and interdigitated by radial tie fiber sheets.152 On top of the superficial layer, there is a ~10 μm thick surface layer of transversely fibrils.151 From the tension-dominated outer to the compression-dominated inner region, the fibers become progressively less aligned, along with an increase in proteoglycan and type II collagen.153,154 (b) IF-guided AFM-nanoindentation on porcine meniscus vertical cryo-sections distinguishes the modulus heterogeneity from inner to outer zones and between T/IT-ECM and PCM (mean ± STD). Groups not connected by the same letter are statistically significant (p < 0.05). Adapted with permission from ref 159. Copyright 2013 Osteoarthritis Research Society. (c) AFM-nanoindentation on bovine meniscus vertical and horizontal cryo-sections distinguishes the modulus zonal heterogeneity for different ECM structural units (*p < 0.001, ns: not significant). (d) AFM-nanoindentation on murine meniscus surface. Left panel: representative indentation force versus depth (F–D) curve shows a non-Hertzian, tension-driven linear F–D dependence. Right panel: murine meniscus surface shows higher modulus (n = 13) than condyle cartilage surface (n = 6) (mean ±95% CI, *p < 0.0001). Adapted with permission from ref 165. Copyright 2015 Elsevier Ltd. Panels a and c are adapted with permission from ref 160. Copyright 2017 Acta Materialia Inc.

Article Snippet: 157 , 158 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7. caption a7 Nanomechanics of the meniscus ECM. (a) Schematic of the hierarchical structure of the meniscus ECM.

Techniques:

Journal: Acta biomaterialia

Article Title: Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears

doi: 10.1016/j.actbio.2018.06.021

Figure Lengend Snippet: Cell migration in injured meniscus explants cultured with inserted DMS.

Article Snippet: Meniscus explants Fresh bovine menisci (medial and lateral) were obtained from normal knees of 18-30 months old animals (Animal Technologies Inc., Tyler, TX).

Techniques: Migration, Cell Culture

Journal: Acta biomaterialia

Article Title: Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears

doi: 10.1016/j.actbio.2018.06.021

Figure Lengend Snippet: PDGFRβ positive cells in injured meniscus explants.

Article Snippet: Meniscus explants Fresh bovine menisci (medial and lateral) were obtained from normal knees of 18-30 months old animals (Animal Technologies Inc., Tyler, TX).

Techniques:

Journal: Acta biomaterialia

Article Title: Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears

doi: 10.1016/j.actbio.2018.06.021

Figure Lengend Snippet: Migratory and proliferating cells in injured meniscus explants.

Article Snippet: Meniscus explants Fresh bovine menisci (medial and lateral) were obtained from normal knees of 18-30 months old animals (Animal Technologies Inc., Tyler, TX).

Techniques:

Journal: Acta biomaterialia

Article Title: Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears

doi: 10.1016/j.actbio.2018.06.021

Figure Lengend Snippet: ECM formation in the injured meniscus explants.

Article Snippet: Meniscus explants Fresh bovine menisci (medial and lateral) were obtained from normal knees of 18-30 months old animals (Animal Technologies Inc., Tyler, TX).

Techniques:

Journal: Acta biomaterialia

Article Title: Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears

doi: 10.1016/j.actbio.2018.06.021

Figure Lengend Snippet: Mechanical properties of the injured meniscus explants cultured with DMS.

Article Snippet: Meniscus explants Fresh bovine menisci (medial and lateral) were obtained from normal knees of 18-30 months old animals (Animal Technologies Inc., Tyler, TX).

Techniques: Cell Culture

Journal: Photonics research

Article Title: Deep Compressed Imaging via Optimized-Pattern Scanning

doi: 10.1364/prj.410556

Figure Lengend Snippet: Detailed parameters of the optical components used in the imaging system.

Article Snippet: The parameters of the lenses used in the setup are listed in . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Element Manufacturer Part Number Note Focusing lens Thorlabs A397TM-A Aspherical lens, focal length 11 mm Pinhole Thorlabs P30D 30 μm pinhole Collimating lens Thorlabs AC254–100-AB-ML Achromatic lens, focal length 100 mm (for pattern scanning) Thorlabs ACL5040-A Aspherical lens, focal length 40 mm (for point scanning) Tube lens Thorlabs SM2V10 Focal length 200 mm Objective lens Olympus RMS4X 4 × objective lens Cylindrical lens Throlabs LJ1014L1-A Focal length 25.4 mm Throlabs LJ1227L2-A Focal length 6.35 mm Lens 1 and 2 OptoSigma SLB-60–250P Focal length 250 mm Relay lens set [56] (one set listed here; two sets arranged symmetrically are required in setup) Throlabs LC1582-A Focal length -75 mm Thorlabs LC1582-A Focal length -75 mm Thorlabs LE1076-A Meniscus Lens, focal length 100 mm Thorlabs LA1399-A Focal length 175 mm Thorlabs LA1050-A Focal length 100 mm Thorlabs LA1727-A Focal length 750 mm Collection lens Thorlabs AC508–075-A-ML Achromatic lens, focal length 75 mm Galvometric scanner Cambridge Technology 6215HM40B Resonant scanner Cambridge Technology CRS 8 KHz Resonant frequency 8 KHz DMD Texas Instruments DLP7000 Open in a separate window Detailed parameters of the optical components used in the imaging system.

Techniques: Imaging

Journal: Cell Transplantation

Article Title: Additional Use of Synovial Mesenchymal Stem Cell Transplantation Following Surgical Repair of a Complex Degenerative Tear of the Medial Meniscus of the Knee: A Case Report

doi: 10.1177/0963689719863793

Figure Lengend Snippet: Above view 3D MR images before and after repair with transplantation of synovial MSCs. Meniscus tear is indicated with arrow. Axial view of 3D MR image is also shown in the inset.

Article Snippet: 3D meniscus images were reconstructed with Ziostation 2 (Ziosoft, Tokyo, Japan).

Techniques: Transplantation Assay

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Continuous Vertical Inside-Out Versus Traditional Vertical Inside-Out Meniscal Repair: A Biomechanical Comparison

doi: 10.1177/23259671231209951

Figure Lengend Snippet: Schematic illustration of the 2 study groups. CS group, with 14 medial menisci, submitted to vertical continuous meniscal repair, and IO group, with 14 medial menisci, submitted to traditional vertical inside-out meniscal repair. The CS group used 4 stitches using the Meniscus 4-ALL device, and the IO group used 4 stitches using the Protector Meniscus device.

Article Snippet: The same suture tape was used in the IO group with the Protector Meniscus meniscal suture device (Arthrex).

Techniques:

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Continuous Vertical Inside-Out Versus Traditional Vertical Inside-Out Meniscal Repair: A Biomechanical Comparison

doi: 10.1177/23259671231209951

Figure Lengend Snippet: Schematic model of continuous vertical meniscal suture. (A) The mini tape of the meniscal suture is pulled out of the joint, removing it from the lumen of the device. (B) Final configuration of a suture with 4 points, with 3 loops and 2 ends of the mini tape suture. (C) Final appearance of continuous vertical suture with 4 points (Images adapted with permission from Rocha de Faria, Pavão DM, Cruz RS, et al. Vertical continuous meniscal suture technique. Arthrosc Tech . 2020;9(9):e1335-e1340. doi:10.1016/j.eats.2020.05.014 et al).

Article Snippet: The same suture tape was used in the IO group with the Protector Meniscus meniscal suture device (Arthrex).

Techniques: